Research Associate Professor, Aquatic & Fishery Sciences
Visiting Scientist, JISAO, NOAA Response & Recovery, Seattle, WA
herwig@uw.edu, 206-685-2163

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aquatic microbiology, microbial ecology and diversity, bioremediation

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Publications

Selected Publications

Dyksterhouse, S.E., J.P. Gray, R.P. Herwig, J.C. Lara, and J.T. Staley. 1995. Cycloclasticus pugetii, gen. nov., sp. nov., an aromatic hydrocarbon-degrading bacterium from marine sediments. Int. J. System. Bacteriol. 45: 116-123. 

Puhakka, J.A., R.P. Herwig, P.M. Koro, G.V. Wolfe, and J.F. Ferguson. 1995. Biodegradation of chlorophenols by mixed and pure cultures from a fluidized-bed reactor. Appl. Microbiol. Biotechnol. 42: 951-957.

Venkatasan, M.I., G. Clevanger, R.P. Herwig, and J.T. Staley. 1995. Chemistry and microbiology of fin whale digestive tract in the generation of fecal sterols, p. 926-928. In J.O. Grimalt and C. Dorronsoro (eds.)., Organic geochemistry: developments and applications to energy, climate, environment and human history. A.I.G.O.A.

Geiselbrecht, A.D., R.P. Herwig, J.W. Deming, and J.T. Staley. 1996. Enumeration and phylogenetic analysis of PAH-degrading marine bacteria from Puget Sound sediments. Appl. Environ. Microbiol. 62: 3344-3349.

Gray, J.P., and R.P. Herwig. 1996. Phylogenetic analysis of the bacterial communities in marine sediments. Appl. Environ. Microbiol. 62: 4049-4059.

Ederer, M.M., R.L. Crawford, R.P. Herwig, and C.S. Orser. 1997. PCP degradation is mediated by closely related species of the genus Sphingomonas. Molecular Ecology 6: 39-49.

Gosink, J.J., R.P. Herwig, and J.T. Staley. 1997. Octadecobacter arcticus gen. nov., sp. nov., and O. antarcticus, sp. nov., nonpigmented, psychrophilic gas vacuolate bacteria from polar sea ice and water. Int. J. System. Bacteriol. 20: 356-365.

Herwig, R.P., J.P. Gray, and D.P. Weston. 1997. Antibacterial resistant bacteria in surficial sediments near salmon net-cage farms in Puget Sound, Washington. Aquaculture 149: 263-283.

Herwig, R.P., and J.P. Gray. 1997. Microbial response to antibacterial treatment in marine microcosms. Aquaculture 152: 139-154.

Walter, G.A., S.E. Strand, R.P. Herwig, T.P. Treat, and H.D. Stensel. 1997. Sustaining TCE degradation by methanotrophic enrichments: TCE and methane feeding strategies. Water Environ. Res. J. 69:1066-1074.

Rockne, K.J., H.D. Stensel, R.P. Herwig, and S.E. Strand. 1998. Cometabolic enhancement of PAH degradation by marine methanotrophic enrichment cultures. Bioremediation J. 1: 209-222.

Shaw, T.R., R.P. Herwig, and J. Wakeman. 1999. Microbial degradation of intertidal creosote-Wyckoff/Eagle Harbor Superfund Site, p. 315-319. Bioremediation technologies for polycyclic aromatic hydrocarbon compounds: proceedings from the Fifth International In Situ and On-Site Bioremediation Symposium, San Diego, April 19-22, 1999.

Fritsche, T.R., M. Horn, M. Wagner, R.P. Herwig, K.-H. Schleifer, and R.K. Gautom. 2000. Phylogenetic diversity among geographically dispersed Chlamydiales endosymbionts recovered from clinical and environmental isolates of Acanthamoeba spp. Appl. Environ. Microb. 66: 2613-2619.

Herwig, R.P. 2000. Pathogens transmitted by seafoods, p. 109-182. In Y.H. Hui, D. Kitts, and P.S. Standfield (ed.), Foodborne Disease Handbook, second edition: volume 4, Seafood and Environmental Toxicants. Marcel Dekker: New York.

Sun, T., G.M. Pigott, and R.P. Herwig. 2002. Lipase-assisted concentration of n-3 polyunsaturated fatty acids from viscera of farmed Atlantic salmon (Salmo salar L.). J. Food Sci. 67:130-136.

Dong, F.M., A.R. Cook, and R.P. Herwig. 2003. High hydrostatic pressure treatment of finfish to inactivate Anisakis simplex J. Food Protect. 66:1924-1926.

Estes, R.M., C.S. Friedman, R.A. Elston, R.P. Herwig. 2004. Pathogenicity testing of shellfish hatchery bacterial isolates on Pacific oyster Crassostrea gigas larvae. Dis. Aquat. Org. 58:223-230.

Purcell, M.K., G. Kurath, K.A. Garver, R.P. Herwig, J.R. Winton. 2004. Quantitative expression profiling of immune response genes in rainbow trout following infectious haematopoietic necrosis virus (IHNV) or DNA vaccination. Fish Shellfish Immunol. 17:447-462. 

Herwig, RP, JR Cordell, JC Perrins, PA Dinnel, RW Gensemer, WA Stubblefield, GM Ruiz, JA Kopp, ML House, WJ Cooper. 2006. Ozone treatment of ballast water on the oil tanker S/T Tonsina: chemistry, biology and toxicity. Mar. Ecol. Prog. Ser. 324:37-55.

Matousek, RC, DW Hill, RP Herwig, JR Cordell, BC Nielsen, NC Ferm, DJ Lawrence, JC Perrins. 2006. Electrolytic sodium hypochlorite system for treatment of ballast water. J. Ship Prod. 22:160-171.

Perrins, JC, WJ Cooper, J van Leeuwen, RP Herwig. 2006. Ozonation of seawater from different locations: formation and decay of total residual oxidant—implications for ballast water treatment. Mar. Poll. Bull. 52:1023-1033.

Perrins, JC, JR Cordell, NC Ferm, JL Grocock, RP Herwig. 2006. Mesocosm experiments for evaluating the biological efficacy of ozone treatment of marine ballast water. Mar. Poll. Bull. 52:1756-1767.

Purcell, MK, KM Nichols, JR Winton, G Kurath, GH Thorgaard, P Wheeler, DJ Hansen, RP Herwig, LK Park. 2006. Comprehensive gene expression profiling following DNA vaccination of rainbow trout against infectious hematopoietic necrosis virus. Molec. Immunol. 43:2089-2106.

Selected Meeting Abstracts

Herwig, R.P., J. L. McLarnan and J.P. Gray. 1998. Composition of microbial communities in contaminated marine sediments. Eighth International Symposium on Microbial Ecology. (Halifax, Nova Scotia, Canada) 

Shaw, T.C., R.P. Herwig, and J. S. Wakeman. 1999. Microbial degradation of intertidal creosote--Wyckoff/Eagle Harbor Superfund Site. In Situ and On-Site Bioremediation Conference. (San Diego) 

Anders, K.M., R.P. Herwig, J.L. McLarnan, R.A. Sanford, and J.F. Ferguson. 1999. Preliminary characterization of O-demethylating and trichlorophenol-dechlorinating marine enrichments. American Society for Microbiology, Annual Meeting. (Chicago). 

Pietari, J.M., R.P. Herwig, J.L. McLarnan, and J.F. Ferguson. Characterization of 10°C groundwater enrichment cultures that dechlorinate PCE and TCE. 1999. American Society for Microbiology, Annual Meeting. (Chicago). 

Herwig, R.P. and J.L. McLarnan. Phylogenetic analysis of microbial communities in creosote-contaminated marine sediments. 1999. American Society for Microbiology, Annual Meeting. (Chicago).

Herwig, R.P., R.M Estes, C.L. Messey, and D.P. Cheney. 1999. Distribution of Vibrio parahaemolyticus in Puget Sound oysters, water, and sediments: preliminary results using a molecular method. National Shellfisheries Association (Pacific Coast Section) and Pacific Coast Shellfish Growers Association, Annual Meeting. (Vancouver, WA). 

Elston, R., A. Gee, and R.P. Herwig. 2000. Bacterial diseases of shellfish seed. National Shellfisheries Association, Annual Meeting. (Seattle). 

Herwig, R.P., Estes, R.M., C.M. Messey, and D.P. Cheney. 2000. Distribution of Vibrio parahaemolyticus in Puget Sound oysters, water and sediments during summer 1999. National Shellfisheries Association, Annual Meeting. (Seattle). 

Herwig, R.P., and D.P. Cheney. 2000. Vibrio parahaemolyticus in Puget Sound, Washington. Pacific Rim Shellfish Sanitation Conference 2000. (Astoria, Oregon) 

Herwig, R.P. 2000. Role of bacteria in the nutrition of bivalve molluscs: conflicting results and research possibilities. August 2000. Microbial Approaches to Aquatic Nutrition within Environmentally Sound Aquaculture Production Systems. Aquaculture Interchange Program Workshop, Center for Applied Aquaculture, The Oceanic Institute. (Honolulu). 

Pietari, J.M.H., R.P. Herwig, and J.F. Ferguson. June 2001. Characterization of tetrachloroethene (PCE) and cis-1,2-dichloroethene (cDCE) dechlorinating cultures with terminal restriction fragment length polymorphism (T-RFLP) analysis. (San Diego).

Estes, R.M., R.P. Herwig, and R.E. Elston. September 2001. Characterization of pathogenic and non-pathogenic bacteria associated with bivalve larvae and shellfish hatcheries. National Shellfisheries Association (Pacific Coast Section) and Pacific Coast Shellfish Growers Association, Annual Meeting. (Silverdale, WA).

Marshall, C.S., and R.P. Herwig. September 2001. Research to examine use of high hydrostatic pressure to inactivate human enteric viruses in oysters. National Shellfisheries Association (Pacific Coast Section) and Pacific Coast Shellfish Growers Association, Annual Meeting. (Silverdale, WA)

Cooper, W.J., J.R. Cordell, P.A. Dinnel, R.W. Gensemer, R.P. Herwig, J.A. Kopp, G.M Ruiz, G. Sonnevil, W.A. Stubblefield, E. VanderWende. February 2002. Ozone, seawater and aquatic nonindigenous species: testing a full-scale ozone ballast water treatment system on an American oil tanker. 11^th International Conference on Aquatic Invasive Species. (Alexandria, VA).

Pietari, J.M.H., R.P. Herwig, and J.F. Ferguson. May 2002. Characterization of a microaerophilic and psychrotrophic tectrachloroethene to cis-1,2-dichloroethene dechlorinating bacterium, strain JPD-1. American Society for Microbiology, Annual Meeting. (Salt Lake City).

Pietari, J.M.H., R.P. Herwig, and J.F. Ferguson. November 2002. Enrichment and Characterization of PCE or cDCE dechlorinating microcosms with PCR and T-RFLP. Superfund Basic Research Program 2002 Annual Meeting. (Tucson, AR).

Herwig, R.P., J.C. Perrins, J.R. Cordell, P.A. Dinnel, R.W. Gensemer, G.M. Dethloff, G.M. Ruiz, W.J. Cooper. March 2003. Ballast water treatment experiments with ozone on board the oil tanker S/T Tonsina. Third International Conference on Marine Bioinvasions. (La Jolla, CA).

Perrins, J.C., and R.P. Herwig. March 2003. Ozone treatment of seawater mesocosm experiments: bacterial community enumeration with flow cytometry . Third International Conference on Marine Bioinvasions. (La Jolla, CA).

Paulson, B.A., E.R. Blatchley III, and R.P. Herwig. March 2003. Effect of ultraviolet light treatment on heterotrophic marine bacteria - evaluation of a technology to treat ballast water in ships. Third International Conference on Marine Bioinvasions. (La Jolla, CA).

Cordell, J.R., C.D. Levings, R.P. Herwig, S. Ong, G.E. Piercey, and J.C. Perrins. March 2003. The origin and consequences of zooplankton arriving in Vancouver, Canada, and Puget Sound, U.S.A. Third International Conference on Marine Bioinvasions. (La Jolla, CA).

Perrins, J.C., and R.P. Herwig. April 2003. Ozone treatment of ballast water for non-indigenous species on board the S/T Tonsina: bacterial enumeration via flow cytometry. Pacific Northwest Chapter of the Society for Environmental Toxicology and Chemistry (PNW-SETAC). (Port Townsend, WA).

Estes, R.M., C.S. Friedman, R.A. Elston, and R.P Herwig. October 2003. Pathogenic and non-pathogenic bacteria associated with Pacific oyster larvae, Crassostrea gigas, and shellfish hatcheries.  National Shellfisheries Association (Pacific Coast Section) and Pacific Coast Shellfish Growers Association, Annual Meeting.  (Portland, OR).

Herwig, R.P. October 2003. Exploring ballast water treatment options: Controlling introductions of non-indigenous aquatic organisms. Sixth Biennial State of the Estuary Conference. (Oakland, CA)

Herwig, R.P. and J.R. Cordell. January 2004. University of Washington Research with SeaKleen®. Environmental Soundness Workgroup, Washington Department of Fish and Wildlife. (Seattle, WA)

Dong, F.M., A.R. Cook, and R.P. Herwig. June 2004. High pressure processing to inactivate Anisakis simplex in raw fish destined for the sashimi market. Institute of Food Technologists (IFT), Annual Meeting. (Las Vegas).

Fraser, W., H.-M. A. Viernes, and R.P. Herwig. November 2004. Quantitation of selected microbial populations in chloroethene-contaminated groundwater. Superfund Basic Research Program 2004 Annual Meeting. (Seattle, WA).

Herwig, R.P., J.C. Perrins, J.R. Cordell, N.C. Ferm, P.A. Dinnel, G.M. Ruiz, R.W. Gensemer, and W. J. Cooper. November 2004. Shipboard testing of a prototype ballast water treatment system: experiments on the West Coast with ozone. Fourth SETAC World Congress. Society for Environmental Toxicology and Chemistry (SETAC). (Portland, OR).

Herwig, R.P., J.R. Cordell, J.C. Perrins, N.C. Ferm, J.L. Grocock, and E.R. Blatchley III. November 2004.  Mesocosm experiments for evaluating potential ballast water treatment systems. Fourth SETAC World Congress. Society for Environmental Toxicology and Chemistry (SETAC). (Portland, OR).

Cordell, J.R., N.C. Ferm, J.L. Grocock, J.C. Perrins, and R.P. Herwig. November 2004. Examination of mesozooplankton present in ballast water of ships entering Puget Sound, Washington. Fourth SETAC World Congress. Society for Environmental Toxicology and Chemistry (SETAC). (Portland, OR).

Perrins, J.C., and R.P. Herwig. November 2004. Flow cytometry versus other methods for enumerating microorganisms present in ballast water. Fourth SETAC World Congress. Society for Environmental Toxicology and Chemistry (SETAC). (Portland, OR).

Young, H. J., J.C. Perrins, R.P. Herwig, and W. J. Cooper. November 2004. Effects of ozonation on CDOM in ballast water. Fourth SETAC World Congress. Society for Environmental Toxicology and Chemistry (SETAC). (Portland, OR).

Lawrence, D., and R.P. Herwig. March 2005. Enumerating phytoplankton abundance in ballast water treatment experiments. 2005 Puget Sound Georgia Basin Research Conference. (Seattle, WA).

Perrins, J., and R.P. Herwig. March 2005. Analysis of ozone and chlorine disinfectant by-products concerning ballast water treatment. 2005 Puget Sound Georgia Basin Research Conference. (Seattle, WA).

Nielsen, B., and R.P. Herwig. March 2005. Efficacy of an on-site filtration and chlorination ballast water treatment system. 2005 Puget Sound Georgia Basin Research Conference. (Seattle, WA).

Herwig, R.P., and J.R. Cordell. March 2005. Defending Our Shores: Ballast Water Treatment Technologies. 2005 Puget Sound Georgia Basin Research Conference. (Seattle, WA).

Lawrence, D.J., and R.P. Herwig. October 2005. Efficacy testing of ballast water treatment technologies: preventing non-indigenous phytoplankton introductions. The Third Symposium on Harmful Algae in the U.S. (Asilomar, CA).